Deciphering the bifidobacterial population within the canine and feline gut microbiota

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During the course of evolution, dogs and cats have been subjected to extensive domestication becoming the principal companion animals for humans. For this reason, their health care, including their intestinal microbiota, is considered of considerable importance. However, the canine and feline gut microbiota is still a largely unexplored research area. In the current work, we profiled the microbiota of 23 feline fecal samples by 16S rRNA gene and bifidobacterial ITS approaches and compared this information with previously reported data from 138 canine fecal samples. The obtained data allowed the reconstruction of the core-gut microbiota of the abovementioned samples coupled with their classification into distinct Community State Types at both genus and species-levels, identifying Bacteroides, Fusobacterium and Prevotella 9 as the main bacterial components of the canine and feline gut microbiota. At species-level, the intestinal bifidobacterial gut community of dogs and cats differed from each other both in terms of species number and composition, as emphasized by a co-variance analysis. Together our findings show that the intestinal populations of cats and dogs are similar in terms of genus-level taxonomical composition, while at bifidobacterial species-level, clear differences were observed, indicative of host-specific colonization behavior by particular bifidobacterial taxa.

Importance Currently, domesticated dogs and cats are the most cherished companion animals for humans and concerns about their health and well-being are therefore important. In this context, the gut microbiota plays a crucial role in maintaining and promoting host health. However, despite the social relevance of domesticated dogs and cats, their intestinal microbial community is still far from being completely understood. In this study, the taxonomical composition of canine and feline gut microbiota was explored at genus- and bifidobacterial-species levels, allowing classification of these microbial populations into distinct Gut Community State Types at either of the two investigated taxonomic levels. Furthermore, the reconstruction of core gut microbiota coupled with co-variance network analysis based on bifidobacterial ITS profiling revealed differences in the bifidobacterial composition of canine and feline gut microbiota, suggesting that particular bifidobacterial species have developed a selective ability to colonize a specific host.

Authors: Giulia Alessandri, Christian Milani, Leonardo Mancabelli, Giulia Longhi, Rosaria Anzalone, Gabriele Andrea Lugli, Sabrina Duranti, Francesca Turroni, Maria Cristina Ossiprandi, Douwe van Sinderen, Marco Ventura
Applied and Environmental Microbiology Jan 2020, AEM.02875-19;
DOI: 10.1128/AEM.02875-19
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