New Vitamin Producing Pediococcus acidilactici Strain Recovered from Gut Microbiota Inhibits Acetic Acid-Induced Colitis

Rats colon segment showing the histo morphological structures differences among the groups

Rat’s colon segment showing the histo-morphological structures differences among the groups.

News Release, International Society of Microbiota - September 22, 2022.

Mansour et al. aimed to isolate, identify and characterize probiotic bacteria as vitamin producers in particular B2 and B9.

  • 150 human fecal samples were collected and used for isolation of vitamin producers—probiotics.
  • 49 isolates were chosen for screening their genome by PCR for the presence of riboflavin and folic acid genes.

As a result, three isolates were selected and their production of the B2 and B9 were confirmed by HPLC. The three isolates were identified on species level by sequencing their 16S rRNA gene which showed 100% identical to strains of Pediococcus acidilactici. They were named as Pacidilactici WNYM01, Pacidilactici WNYM02, Pacidilactici WNYM03 and submitted to the Genbank database with accession numbers.

They met the probiotic criteria by expressing 90–95% survival rate at pH (2.0–9.0) and bile salt up to 2% for 3 h in addition to their antimicrobial activity against gram positive and negative microorganisms. They also showed no hemolytic activity and common pattern for antibiotic susceptibility.

The three strains were tested individually or in mixture in vivo on rat colitis model compared to ulcerative group. They were administrated orally to rats in daily dose containing CFU 109 for 14 days then followed by induction of colitis using acetic acid then the oral administration was continued for more four days.The histology results, the anti-inflammatory and anti-oxidative stress biomarkers showed the protective role of the strains compared to the ulcerative group.

In conclusion this work introduced novel three probiotic candidates for pharmaceutical preparations and health applications.

Article DOI.

© Image - Mansour et al.

Dr. Mansour will be joining Targeting Microbiota 2022 to share her latest research on gene editing and microbiome therapy

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